Research Associate - In Vitro Models
- Conduct iPSC culture, expansion and directed differentiation workflows
- Perform routine and specialized mammalian cell culture, including maintenance and expansion of various cell lines
- Maintain consistent cell quality and optimize cell culture protocols to ensure reproducibility across experiments
- Collaborate with team members to develop standard operating procedures (SOPs) for scaling disease-relevant in vitro tissue models
- Implement protocols for processing and dissociation of various primary tissues
- Execute molecular biology assays for model characterization, including qPCR, Western blotting, ELISA, flow cytometry, FACS, and immunocytochemistry
- Implement multi-modal bulk and single cell assays as an integral part of testing workflows
- Maintain detailed, well-organized documentation of experiments and data
- M.S. or B.S. with 2+ years industry experience in a relevant life science field (cell biology, molecular biology, bioengineering, biomedical sciences)
- 3+ years of hands-on experience in the lab, with demonstrated proficiency in mammalian cell culture across cancer cell lines, primary cells, and stem cells
- 2+ years of hands-on experience with iPSC maintenance and differentiation
- Proficiency with basic molecular biology techniques (i.e. RNA extractions, qPCR, Western blotting, immunocytochemistry, microscopy, flow cytometry, ELISA)
- Detail-oriented, with strong organizational and record-keeping skills
- Excellent communication skills and ability to work both independently and collaborate effectively in a fast-paced research environment
- High motivation with a strong work ethic and dedication to generating impact
- Preferred:
- Prior experience working with 3D cell culture models or tissue engineering
- Experience with cell sorting and processing primary tissue for various workflows (i.e. preparation of single-cell suspensions for cell-culture, flow cytometry, FACS, scRNA-seq)
- Familiarity with high-throughput screening systems, shake flasks, or bioreactors
- Experience contributing to the development of disease models for therapeutic testing
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